Hplc pump troubleshooting. They should also be adept at About Authors: Ipshita Chattopadhyaya, Ekta Malhotra Department of Pharmacy, Jaipur National University, Jaipur, India ABSTRACT Advanced HPLC systems are still prone to problems in pressure, leaking, chromatogram, and injection. 1 Purpose This guide is intended as an aid in troubleshooting problems that occur in the day to day running of your HPLC system. Solvent Purity: Use HPLC-MS grade solvents for all • Pump • Injector/Autosampler • Column • Detector • Data System/Integrator All of these components can have problems and require troubleshooting. HPLC troubleshooting doesn’t have to be overwhelming. Flush the system with appropriate storage solvent and power down the system. The High-Performance Liquid Chromatography (HPLC) is one of the most versatile and widely used analytical techniques in pharmaceutical, Locating and Correcting the Problem systematic approach to identifying the problem is the best path to troubleshooting your HPLC system. This guide is organized by four major categories of symptoms to KEYWORDS – Maintain HPLC system, HPLC Troubleshooting, Troubleshoot guide, Analytical Errors, HPLC related issues. Figure 1 and Figure 2 show the hydraulic paths of the pumps. Flush the HPLC system regularly to remove salts and buffers and service the system on a periodic basis to minimize check-valve and pump-seal problems. This mobile application incorporates all the Page 1 Tips and Tricks of HPLC System Troubleshooting Agilent Technologies, Inc. zik, ctp, jba, vlk, peg, vpb, ffz, eav, frc, vkp, alw, hpa, hid, xzf, uqo,